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Description
FITC Mouse Anti-Human CD133 Antibody (S-R586)Product Specification Host Mouse Antigen CD133 Synonyms Prominin 1; Antigen AC133; Prominin like protein 1; PROML1; PROM1 Location Cell membrane, Endoplasmic reticulum Accession O43490 Clone Number S R586 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Positive Sample human PBMC Purification Protein G Concentration 0. 05mg ml Conjugation FITC Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin 300
Product Specification
| Host | Mouse |
| Antigen | CD133 |
| Synonyms | Prominin-1; Antigen AC133; Prominin-like protein 1; PROML1; PROM1 |
| Location | Cell membrane, Endoplasmic reticulum |
| Accession | O43490 |
| Clone Number | S-R586 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | FITC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD133, also known as prominin-1, is a pentaspan transmembrane glycoprotein encoded by the PROM1 gene. It is widely used as a biomarker for the identification and isolation of stem and progenitor cells in various normal tissues and cancer stem cells (CSCs) in different types of tumors. CD133 has five transmembrane domains, with its N-terminus extending into the extracellular space and the C-terminus residing inside the cell. It is expressed in neural progenitor cells, hematopoietic cells, and various cancer cells, including those in brain, colon, and pancreatic tumors. The presence of CD133 is associated with self-renewal, tumorigenicity, and resistance to chemotherapy and radiotherapy, making it a significant marker for cancer prognosis and therapeutic targeting.
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